Unmethylated Vitamin D Receptor (VDR) pada Multi Drug Resistant Tuberculosis

Abstract

Background : Cases of Tuberculosis (TB) and multi-drug resistant Tuberculosis (MDR TB) caused by mycobacterium tuberculosis are high globally. Vitamin D has an important role against TB infection by inducing antimicrobial peptides such as cathelicidin through vitamin D receptors (VDR). Several studies suggest that there is a genetic influence for TB infection, such as polymorphism and methylation. Methylation of VDR gene is one of the causes of susceptibility to TB infection. Objective : This study aimed to describe the unmethylated vitamin D receptor gene in patients with MDR TB and pulmonary non-MDR TB in Medan City. Methods : This research was a descriptive studied with a cross sectional research design. Stored DNA sample isolated from blood 40 patient MDR TB blood and 40 patient non MDR TB was converted to bisulfite with EZ DNA Methylation-Gold Kit. Converted DNA was amplified by PCR and finally gel electrophoresis was performed with agarose gel to see the unmethylated. Results : Unmethylated VDR gene was higher in MDR TB (17,5%) than in non MDR TB (null). Unmethylated VDR gene was found higher in men (15%) and in age 18-49 (10%). Conclusion : There was no unmethylated VDR gene in non MDR TB. The unmethylated VDR gene was found in MDR TB.