The Effects of Phaleria macrocarpa (Scheff.) Boerl Extract on Tumor Necrosis Factor-Alpha (TNF-α) Level in Preeclampsia-Induced Human Umbilical Vein Endothelial Cell (HUVEC) Culture

Abstract

Preeclampsia is a major cause in both maternal and perinatal mortality and morbidity. The etiopathogenesis of preeclampsia is still not fully elucidated but it is believed to be a multifactor. Endothelial dysfunction plays a big role in the pathophysiology of preeclampsia. Tumor Necrosis Factor-α (TNF-α) is considered as one of the potentially specific markers for preeclampsia. In vitro model research is considered the best and most effective way to understand the disease pathophysiology. HUVEC (Human Umbilical Vein Endothelial Cell) culture is an in vitro model widely used to study the pathogenesis of preeclampsia. Phaleria macrocarpa (Scheff.) Boerl also known as Mahkota Dewa is widely used as an anti-inflammation and antioxidant because of alkaloids, saponins, flavonoids and polyphenols properties. This study aimed to determine the effects of Phaleria macrocarpa Extract on Tumor Necrosis Factor – Alpha (TNF- α) Level In preeclampsia-induced Human Umbilical Vein Endothelial Cell (HUVEC). Our results showed the Phaleria macrocarpa’s extract reduce TNF-α level siginifcantly at concentration of 7.813 μg/mL. Phaleria macrocarpa’s extract at concentration of 62.5 μg/mL reduce TNF-α level to normal level. Thus, Phaleria macrocarpa’s extract might be used as agent to overcome endothelial dysfunction in preeclampsia.