Polimorfisme Gen Vitamin D Receptor (VDR) BsmI pada Multi Drug Resistant Tuberculosis (MDR TB)

Abstract

Background : The increasing cases of Tuberculosis (TB) is a concerned health problem to the world, especially Indonesia, which Indonesia is the 2nd most TB cases in the world. The increase in TB cases is accompanied by the increase in Multi Drug Resistant Tuberculosis (MDR TB) cases. Genetic and immunity host factor, inadequate treatment and bacterial mutations affect the infection of MDR TB. Vitamin D has an important role in immune regulatory to eliminate Mycobacterium tuberculosis, as of it can affect the susceptibility to MDR TB. Purpose : The aim of this study was to determine the polymorphism of the Vitamin D Receptor (VDR) BsmI gene in MDR Tuberculosis. Methods : This study was a case control study involving 32 non-MDR TB patients and 32 MDR TB patients. The VDR gene was restricted by the Restriction Fragment Length Polymorphism (RFLP) method using BsmI enzyme and agarose gel visualization was performed. Results : Homozygous variant GG genotype was found higher in non-MDR Tuberculosis (43.75%) than in MDR Tuberculosis (18.8%), and heterozygotes AG genotype was not found in MDR Tuberculosis either in non MDR Tuberculosis. There was no significant relationship between the VDR BsmI gene polymorphism and MDR tuberculosis (p>0.05). Conclusion : This results showed that BsmI VDR gene polymorphism does not affect the susceptibility to MDR Tuberculosis.